Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Using alkaline single-cell gel electrophoresis (the Comet assay), we show that expression of PrPC protects human neuroblastoma SH-SY5Y cells against DNA damage under basal conditions and following exposure to reactive oxygen species, either hydroxyl radicals following exposure to Cu2+ or Fe2+ or singlet oxygen following exposure to the photosensitizer methylene blue and white light.
|
17697940 |
2007 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
In this issue of the JCI, Pfeifer et al. report that lentivector-mediated RNAi significantly reduced neuronal PrP(C) expression; effectively suppressed accumulation of the infectious protease-resistant form of PrP (PrP(Sc)) in a persistently infected neuroblastoma cell line; and markedly slowed the progression of prion disease in a unique chimeric mouse model (see the related article beginning on page 3204).
|
17143323 |
2006 |
Childhood Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
We have established stably transfected human neuroblastoma cells (SH-SY5Y) expressing mutant V210I, or wild-type PrPc.
|
10454141 |
1999 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In this study, introduction of small interfering (si) and small hairpin (sh) RNAs targeting the prion protein gene (prnp) transcripts triggered specific gene silencing and reduced the PrP(C) level in both prion-free and -infected neuroblastoma cell lines.
|
19748523 |
2009 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Cellular prion protein mediates early apoptotic proteome alternation and phospho-modification in human neuroblastoma cells.
|
28102851 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Here, we show for the first time that ADAM23, which is predominantly expressed in the central nervous system, co-localizes with cellular prion protein (PrP(C)) at plasma membrane of mouse hippocampal neurons and neuroblastoma cells.
|
19477226 |
2009 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Transient expression of cytoplasmic PrP produced juxtanuclear aggregates reminiscent of aggresomes in human embryonic kidney 293 cells, human neuroblastoma BE2-M17 cells and mouse neuroblastoma N2a cells.
|
16696854 |
2006 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
To investigate the downstream mechanism of PrP(C) and Aβ oligomer interaction and their possible relationships to tau, we examined tau expression in human neuroblastoma BE(2)-C cells transfected with murine PrP(C) and studied the effect under Aβ oligomer treatment.
|
23805846 |
2013 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
For an efficient experimental readout, green fluorescent protein (GFP)-tagged mutant prion protein (PrP(187R-GFP)) and wild-type PrP (PrP(C-GFP)) were expressed in human neuroblastoma cells.
|
15046864 |
2004 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Indeed, in vitro studies using N2a neuroblastoma cells demonstrated that inhibition of Grp58 expression with small interfering RNA led to a significant enhancement of PrP(SC) toxicity.
|
15772339 |
2005 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
To understand if the coaggregation of PrPC is governed by PrP mutations or is a consequence of the cellular compartment of PrPM aggregation, we coexpressed PrPM and PrPC in neuroblastoma cells, the latter tagged with green fluorescent protein (PrPC-GFP) for differentiation.
|
12485397 |
2003 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We recently employed quantitative mass spectrometry to characterize the interactome of the prion protein in a murine neuroblastoma cell line (N2a), an established cell model for prion replication.
|
21163327 |
2011 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We used the SH-SY5Y human neuroblastoma cell line and treated the cells with melatonin and then exposed them to the prion protein, PrP, or the β-catenin inhibitor, ICG-001.
|
23900566 |
2013 |
Childhood Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
We have examined the role of molecular chaperones in the folding of normal and mutant PrP Q217R (PrP(217)) in transfected neuroblastoma cells.
|
10970892 |
2000 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We have explored the two-step model in prion-infected mouse neuroblastoma (ScN2a) cells by focusing on the mouse PrP (MoPrP) segment 92-GGTHNQWNKPSKPKTN-107, which is within a region previously suggested to be part of the binding interface or shown to differ in its accessibility to anti-PrP antibodies between PrP(C) and PrP(Sc).
|
22398286 |
2012 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Membrane-enriched proteome changes and prion protein expression during neural differentiation and in neuroblastoma cells.
|
28431525 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Western blot analysis results revealed that these PrP-specific siRNAs could effectively knock down the levels of either endogenous PrP in human neuroblastoma SHSY-5Y cells or recombinant PrP transfected with the plasmid expressing the full-length human PrP in human embryonic kidney (HEK) 293T cells.
|
21537833 |
2011 |